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Culture Media

Mycoplasma Culture Media

We offer three different product lines of mycoplasma culture media:

  • The Product Line Pharma QC for GMP-compliant regulatory mycoplasma testing by the culture methods according to the compendial protocols
  • The Product Line Microbiology for general growth and cultivation of mycoplasmas on small or large scale
  • The Product Line Diagnostics for isolation of mycoplasmas from clinical samples

All media products are ready-to-use and available as liquid media or agar plates. The standard units are 100 mL of liquid media supplied in bottles and packs of 20 poured 60-mm agar plates. For special applications, smaller or larger volumes of liquid media (e.g., 50 mL for mycoplasma testing according to the PTC and Guidance for Industry; 800 mL for sample dilution) or larger agar plates (e.g., for filter challenge studies) can be produced on request. Please contact us (office@mycoplasma.cc) for details. We will make sure to meet your media requirements for your mycoplasma testing needs and are very flexible in terms of production and delivery times.

For each medium lot a certificate of analysis is provided containing the quality control results of mycoplasma growth promotion tests performed according to EP 2.6.7. and USP <63>.

 

mbsFRIIS & mbsFREY

Product Characteristics

  • mbsFRIIS & mbsFREY are standard mycoplasma culture media according to EP 2.6.7. and USP <63>
  • Suitable for regulatory mycoplasma testing by the official culture method
  • Suitable as diagnostic media
  • Suitable as general mycoplasma culture media

 

mbsABO

Product Characteristics

  • A universal mycoplasma culture medium for rapid growth of ALL common cultivable mycoplasmas and of the “non-cultivable” Mycoplasma hyorhinis cultivar-α strains
  • Enables rapid mycoplasma broth enrichment prior to analysis by PCR-based detection assays
  • Suitable for regulatory mycoplasma testing by the conventional culture method
  • Suitable for regulatory mycoplasma testing by combined enrichment/PCR hybrid assays
  • Suitable as diagnostic medium
  • Suitable as general mycoplasma culture medium

Advantages compared to the standard mycoplasma culture media

  • mbsABO supports growth of ALL common cultivable Mycoplasma, Acholeplasma and Spiroplasma species 
  • mbsABO supports growth of the highly fastidious “non-cultivable” cell culture-adapted Mycoplasma hyorhinis cultivar-α strains
  • mbsABO enables increased mycoplasma growth rates which lead to much higher titers after only few days of enrichment than the standard mycoplasma culture media
  • mbsABO allows to replace the indicator cell culture method when used as culture medium for regulatory mycoplasma testing by the conventional culture method

 

mbsALPHA

Product Characteristics

  • A selective culture medium for rapid growth of “non-cultivable” Mycoplasma hyorhinis cultivar-α strains
  • Suitable for CFU quantification of the cultivar α reference strain Mycoplasma hyorhinis DBS 1050 required as positive control strain for mycoplasma testing by the indicator cell culture method
  • Can replace the indicator cell culture method when included as additional medium in regulatory mycoplasma testing by the culture method

 

mbsMHYO

Product Characteristics

  • A non-selective growth medium for “the 3 Mhyo" mycoplasma species, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, and Mycoplasma hyorsynoviae
  • Enables increased formation of M. hyopneumoniae colonies in fried-egg appearance usually not seen in M. hyopneumoniae strains, with colony sizes that allow reliable colony counting and CFU quantification after 10-14 days of incubation
  • Suitable as diagnostic medium for isolation of M. hyopneumoniae, M. hyorhinis, and M. hyorsynoviae
  • Suitable as high-yield culture medium for M. hyopneumoniae, M. hyorhinis, and M. hyorsynoviae

 

mbsUREA

Product Characteristics

  • A selective growth medium for Ureaplasma species
  • Suitable as diagnostic medium for isolation of ureaplasmas
  • Suitable as high-yield culture medium for ureaplasmas

 

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